The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. /525 em. . 9000-0756. Luminescence was immediately measured by a SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA) after mixing the fecal supernatant and Hgb standard with the same amount of luminol reagent. The absorbance change at 620 nm was measured by a SpectraMax M2 Microplate Reader (Molecular Devices). SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. 3, La Jolla, CA, USA). Lift up the lid over the chamber to insert or remove a cuvette. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forSpectraMax® M3. Cells were exposed to drug solutions with. The adherent cells were incubated in 100 μl of lysis buffer on ice for 20 min. The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. Detection modalities are shown in Table 1-1. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. Cells were seeded at 5000 cells well −1 on 96-well plates and allowed to grow for 24 h before treatment. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. normalized response (Graphpad Prism 8. TEM. Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. High tolerance for contaminants. For cell cultures, the medium was removed 24 h after initiation of drug stimulation and frozen for subsequent analysis, with no need for sample dilution. VIEW DETAILS The samples were centrifuged to pellet the remaining intact erythrocytes, and supernatants were collected. When a feature or capability applies to only certain readers, this exception is noted. The fluorescence intensity was measured as ratio of values at an excitation at 340 nm and 380 nm with an emission at 510 nm, using Spectramax M4 (Molecular Devices). MTT was then removed, and the formed formazan crystals were then dissolved in 150‐μl DMSO. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. 025 mol/L sulfuric acid) and then measured following the molybdenum antimony colorimetric method (multifunctional microplate reader, SpectraMax M4, MD company, USA). The optical absorbance of the plate at 450 nm was measured using a multifunctional microplate reader SpectraMax M4 (Molecular Devices, LLC, San Jose, CA, USA). AquaMax® 4000. 2. Background-subtracted data values (mean +/- SD) are graphed. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. neoformans was cultured from glycerol stock in 5 mL of YM broth at 25°C for 72 hours at 200 rpm. . Interpolated concentrations of native PF4 in human serum, plasma and cell culture supernatant samples. SpectraMax ABS Plus SpectraTest ABS2 v1. 2. The absorbance at 550 nm was recorded using a SpectraMax M4 microplate reader (Molecular Devices) following solvation of formazan with 100 μL DMSO. Formazan crystals formed were solubilized in 10 μl DMSO, and absorbance was recorded using a multimode plate reader (SpectraMax M4; Molecular Devices, USA) at 606-nm wavelength. All configurations. The ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. Danaher Inc spectramax m series Spectramax M Series, supplied by Danaher Inc, used in various techniques. Lysosomal proteolytic activity. SpectraMax M Series Multi-Mode Microplate Readers | MolecularThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Hemoglobin release from erythrocyte lysis was detected by measuring the optical density (OD) of the supernatant at 412 nm using a microplate reader (SpectraMax M4 from Molecular Devices, or Tecan SPARK, from Tecan, Männedorf, Switzerland). Colony Formation Assay. ROS estimation by 2′,7′- dichlorofluorescin diacetate (DCFDA) staining . alamarBlue reagent volume in a different spheroid microplate formatA SpectraMax M4 microplate reader (Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at a wavelength of 450 nm. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Description 1. Multiplate reader for ELISA detection (Spectramax M4). FlexStation™ I. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected] number of moles of FITC conjugated to OVA was determined using UV–vis spectroscopy (SpectraMax M4, Molecular Devices Inc. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4To quantify F-CHP fluorescence, stained samples were digested in 500 μl of proteinase K (1 mg/ml) in water for 3 hours at 60°C. org) with optimized instrument settings (Table 1) were used to generate and analyze data. . After 48 h, the optical density (OD) was measured at 450 nm using the microplate reader (SpectraMax M4). SpectraMax® M5e. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. After incubating for 10 min at room temperature, the absorbances of standards and samples were measured at 450 and 590 nm, 12 using a Spectramax M4 spectrophotometer (Molecular Devices). Members of the Sphingomonadales are renowned for their ability to degrade polycyclic aromatic hydrocarbons (PAHs). 1% CF3COOH) = 5/95 (v/v) to 95/5 (v/v); flow rate:The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. ® reader to the computer. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 22 μM was mixed with 10-μl enzyme giving a final enzyme concentration in the. Three kits covering a wide range of dsDNA concentrations. Expand the ABS1 Plate Inserted or Expt#3 experiment and select the OptAlign&UltDrk Plate section. The cleaved substrate was excited at 356-nm, and the fluorescence emissions were detected. e. The following document is intended to demonstrate setup of this instrument. When a feature or capability applies to only certain readers, this exception is noted. 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The optical density at 450 nm was detected using a microplate reader (SpectraMax M4, MolecularDevices, CA, USA). DNA standards ranging from 50 pg/mL to 1 μg/mL were detected using the Quant-iT PicoGreen dsDNA Assay Kit and SpectraMax readers (data from the SpectraMax iD5 reader are shown, but other SpectraMax readers gave similar results). Indie Digital & Print Publishing. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. SpectraMax ABS/ABS Plus SpectraMax i3/i3x MiniMax SpectraMax iD3/iD5 Spectramax 190 EXT Spectramax 340PC/384 Spectramax Plus 384 Spectramax L Spectramax M2 Spectramax M2e Spectramax M3 Spectramax M4 Spectramax M5 Spectramax M5e Spectramax Mini Versamax EXT Vmax Microplate Washers; Instrument Field Depot; Aquamax 2000 Aquamax 2000/Stakmax. The MTT reagent was incubated with cells for 4 h. After aerosol collection was completed, the impactor plates were rinsed with 0. Background-subtracted data values (mean +/- SD) are graphed. Living cells were then incubated with 100 µl of medium assay provided by the assay kit. Table 2. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Prepare 22 mg/mL of FD4, 16 mg/mL of rhodamine70, and 40 mg/mL of creatinine in sterile PBS. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Choose from a three- (M3), four- (M4), orThe optical density was determined spectrophotometrically at 405 nm using a SpectraMax M4 microplate reader (Molecular Devices, Sunnyvale, CA). Data analysis methods. 2. To avoid injury, it is recommended that two. † Bottom mounting for SpectraMax Paradigm Readers only. Note: In this user guide, all references to SpectraMax Multi-Mode. Phillips5, Martin A. 7% of the total test aerosol mass) was not included in the collection analysis. Serial number location on Series II and Multi-Mode plate readers. StakMax Handling Stacker Base Unit. In the competitive field of drug development where timely go/no-go decisions must be made to bring effective class-leading drugs to market, it is vital to work with a CRO that can provide highly. Ninety microliters of 2-chloro-4-nitrophenyl-α- d -maltotrioside substrate dilutions from 5000 to 1. SpectraMax® Paradigm® Multi-Mode Detection Platform. Full content visible, double tap to read brief content. 4) and cells were stained with 150 nM TSQ for 1 min. Preconfigured SoftMax ® Pro Software protocols (available at softmaxpro. D 1 1. Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates LM1, FlexStation 3 reader, SpectraMax L reader, LMax II (Obsolete), SpectraMax M5/M5e reader. , contact Molecular. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Description 1. 6: bioNova científica, s. 90-0083 series): Excitation 530 nm,. With optimized reagents, option to operate the. The chloride and pH. When using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. Hemoglobin release from erythrocyte lysis was detected by measuring the optical density (OD) of the supernatant at 412 nm using a microplate reader (SpectraMax M4 from Molecular Devices, or Tecan SPARK, from Tecan, Männedorf,. Choose from a three- (M3), four- (M4), or Cables. . For labeling representative cells, they were treated under the same conditions as above, followed by fixation with 2% paraformaldehyde for 20 min at room temperature. Rhizosphere soil microbial communities substantially impact plant growth by regulating the nutrient cycle. S. 2. . Images were taken using a fluorescence microscope (Nikon 80i, Japan). Preconfigured SoftMax® Pro Software protocols (available at softmaxpro. 5 mL of creatinine, FD-4, and rhodamine70 dissolved in. We therefore gathered salient. The plate reader optics are tunable for absorbance. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Choose from a three- (M3), four- (M4), orThe absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). Moving a SpectraMax Multi-Mode Microplate Reader. . ATP standard curves read on SpectraMax microplate readers. Assessment of Neurite Outgrowth. The absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). Data are from ≥5 independent experiments. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Plot the fluorescence intensity versus the cell seeding number. Italic and boldface type are used for emphasis. The samples were centrifuged to pellet the remaining intact erythrocytes, and supernatants were collected. . The following document is intended to demonstrate setup of this instrument. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The luciferase values were determined by normalization to Renilla values (as a control) to correct for variation in transfection efficiencies. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). 383 inches whereas the maximum allowed is 5. View specifications, prices, citations, reviews, and more. Click Read. We therefore gathered salient information about many of the. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Finally, we have two New Brunswick Excella E24 shaking incubators, one with refrigeration capabilities and a Thermo Sciences -80˚C freezer. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. The absorbance of solubilized formazan crystals was measured at 570 nm using a multimode reader (Spectramax M4, Molecular Devices LLC, Sunnyvale, CA, USA). book Page 1 Wednesday, July 7, 2010 2:09 PM. *Statistical significance: p < 0. SPECTRAMAX 190 reader: TRUE: Molecular Devices: SPECTRAMAX 340PC/340PC 384 reader: TRUE: Molecular Devices. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Inhibition of CPT-11 Hydrolysis by Four Bioflavonesfor the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Molecular Devices. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader Can you recommend disposable cuvettes for use in the microplate readers? Molecular Devices® does not recommend any specific disposable cuvette brands. CMC values were determined from the pyrene fluorescence in solution as a function of conjugate. Real time thermal cyclers (iCycleriQ apparatus with iCyclerOptical System software version 3. Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politicsSpectraMax Spectrofluorometers. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. Qualify the luminescence performance of the SpectraMax . The filtered samples were transferred to a 96-well plate and the fluorescence was measured using a plate reader (Spectramax ™ M4, Multi detection Reader) at excitation and emission wavelengths of λex 339 and λem 390 nm, respectively. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. 9 pg/mL. 38. FP assay do not perform well with protein:protein interactions. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. C. Test samples were measured according to the manufacturer’s instructions using a spectrophotometer SpectraMax M4 (Molecular Devices, Sunnyvale, CA). The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Dettmer4, Wayne A. Microplate Readers include the M3, M4,. Table of Contents. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. instruments you must first insert an adapter plate. 05, ** p <. ). Results were normalized and expressed as a percentage of live cells. Acridine orange (AO) staining evaluates the morphological changes in apoptotic cells by formation of blebs in the membrane, fragmentation and condensation of the. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. Example: [Drawer]. The 96-well plates were read on the SpectraMax M4 plate reader at the following wavelengths: green fluorescence at 488 ex. Bioz Stars score: 98/100, based on 1 PubMed citations. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. Because of possible losses from settling, particle data for the >7 µm size fraction (<0. All experiments were performed in triplicate. Luminescence was immediately detected by the SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA). Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. FlexStation ®, SpectraMax. The following document is intended to demonstrate setup of this instrument. When using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. Walter2, and Roch-Philippe Charles1 1Institute for Biochemistry and Molecular. neoformans was cultured from glycerol stock in 5 mL of YM broth at 25°C for 72 hours at 200 rpm. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Bioz Stars score: 86/100, based on 1 PubMed citations. The DQ Green BSA probe. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected]. The incubation system pre-incubated at 37°C for 3 min minutes and 2 μL fluorescent substrate FD (5 μM, final concentration) was added for fluorescence analysis in fully automated fluorescent microplate reader (SpectraMax M4, Molecular Devices) (Wang et al. 2. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. 3. 2. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. DNA standards. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. Size and zeta potential of nanoparticles were measured by Zetasizer Nano ZS90 (Malvern Instrument). Italic and boldface type are used for emphasis. 316 grating motor driver faulty. The absorption value at 570 nm was measured using a Molecular Devices SpectraMax M4 plate reader, and the absorbance at 690 nm was subtracted as the background. The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. Hexokinase reagent (Eagle Diagnostics) was added and the reaction absorbance was read at 340 nm using a SpectraMax M4 multimode plate reader (Molecular Devices). Learn how to easily set up an efficient and informative plate read by using SoftMax Pro 6 Microplate Data Acquisition and Analysis software. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Note: In this user guide, all references to SpectraMax Multi-Mode. 315 can't find zero order. Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. Diclofenac potassium at non-toxic concentration on U87-MG was used as positive control. SpectraMax M3, M4, and M5/M5e readers. 2. No. Choose from a three- (M3), four- (M4), or We would like to show you a description here but the site won’t allow us. 4. Brand: Molecular. Fluorescence intensities of each well were examined by a microplate reader (SpectraMax M4, Molecular Devices, CA, USA) with 485-nm excitation and 525-nm emission wavelengths. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. SpectraMax M Series Multi-Mode Microplate Readers | Molecular Devices · 2020-04-14 · use two scanning monochromators so the user can determine optimal excitation and emission settings, of 8 /8 Match case Limit results 1 per pageThe culture medium was removed, and the blue product was dissolved in DMSO (100 μl). The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. N2a cells were induced for neurite growth by adding nerve growth factor (NGF) (50 ng/ml). Measurements of temperature increase (ΔT) during the exposure of MNP/AMF hyperthermiaThe culture medium was removed, and the blue product was dissolved in DMSO (100 μl). /525 em. Plate chamber design of the SpectraMax category 1 microplate readers. 1. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. 2. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. The final concentration of hemoglobin in feces was. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. +1 250-415-0785. Other technologies such as imaging. After treatment, the Cell Counting Kit-8 (CCK-8) (Dojindo Molecular Technologies, Rockville, MD, USA) was added to the PA and skatole treated hepatocytes and additionally incubated for 1 h. Each well was scanned at nine evenly distributed points and averaged automatically with the software. SpectraMax i3(x) SpectaTest ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. SigmaPlot (Systat Software, Inc. 05 mol/L hydrochloric acid and 0. Large molecule bioanalysis, immunogenicity and PK testing with higher quality data, rapid turnaround and fewer surprises. Optimized for fluorescence microplate readers. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. Fifteen different automated tests are provided: • Background noise • Background spike • Lower limit of detection • Crosstalk • Linearity • Relative luminescence units (RLU) • Kinetic noise (low signal)0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. SpectraMax M2/M2e reader: TRUE: Molecular Devices: Aquamax DW4 washer: TRUE: Molecular Devices: SpectraMax M4 reader: TRUE: Molecular Devices: SpectraMax M5/M5e reader: TRUE: Molecular Devices:. Sample Fluorescent Reader Setting for the ADP Hunter™ Plus (Cat. The optical systems Operated from within SoftMax Pro Software, the StakMax Microplate Handler can hold up to 50 plates and facilitates barcode reading. Plate Formats: 6, 12, 24, 48, 96, 384 Well Plates. Background-subtracted data values (mean +/- SD) are graphed. e. Operated from(A) ROS levels and (B) NAD+/NADH ratios in rotenone-pretreated young HDFs. 01) + 0. SpectraMax ABS/ABS Plus SpectraMax i3/i3x MiniMax SpectraMax iD3/iD5 Spectramax 190 EXT Spectramax 340PC/384 Spectramax Plus 384 Spectramax L Spectramax M2 Spectramax M2e Spectramax M3 Spectramax M4 Spectramax M5 Spectramax M5e Spectramax Mini Versamax EXT Vmax Microplate Washers;. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. After 7 days of culture, the colonies generated. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. 1. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Parameter SpectraMax iD5 SpectraMax i3x. The polysaccharide HPA with different concentrations (0, 25, 50, and 100 μg/mL) was added and incubated with cells for 24 h. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Cleavage of the fluorescent NAG substrate (4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-Glucopyranoside) was measured for 20 min with 45 s intervals at 30°C in a Molecular Devices (San Jose, CA) SpectraMax M4 microplate reader. Table 2. to View Figures. 306 plate air cal fail,low light. Statistical. The pathophysiology of such. 116 SpectraMax M5 and M5e Multi-Mode. For heme titration experiments a constant ATP concentration of 1 mM was used. 1 & 7. 1. Supplier: Molecular Devices. Flasks containing 100 mL of YM broth were inoculated with 100. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax Microplate Stacker for walk-away processing. GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable. This platform allows the. We therefore gathered salient. Laboratory Freezers. At sacrifice, serum was isolated from mouse total blood and maintained at –80 °C. High-performance liquid chromatography (HPLC) chromatograms were performed on Agilent Infinity 1260 with a C18 column (100 Å, 30 × 4. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. To determine the cell viability, thiazolyl blue tetrazolium bromide (MTT) assay was performed. Products : SpectraMax M2/M2e reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax 190 reader, VersaMax reader, Gemini EM reader, SpectraMax Plus 384 reader. FD-4 concentrations in plasma were analyzed in duplicates using a spectrophotometer (SpectraMax M4, Molecular Devices, San Jose, CA, USA) with excitation λ 485 nm and emission λ 535 nm. Fifteen different automated tests are provided: • Background noise • Background spike • Lower limit of detection • Crosstalk • Linearity • Relative luminescence units (RLU) • Kinetic noise (low signal) 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. 7. ” The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. M2 M2e M3 M4 M5 M5e ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. 5. For SpectraMax L or LMax II, LMax II 384 - no adapter used. , 2011). Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. SpectraMax® iD5. Background-subtracted data values (mean +/- SD) are graphed. Large Molecule Bioanalysis. * Nunc. The absorbance was determined by a microplate reader (SpectraMax M4, USA) at 405 nm. Shop our huge selection of OEM, MRO, construction, industrial, and safety products. 116 SpectraMax M5 and M5e Multi-Mode. RFU was plotted versus time to derive the rate of formation of the fluorophore. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 1, BIO-RAD Laboratories). Table of Contents. If using the StakMax and a SpectraMax reader together, the time specifications for the movement of the. Where is the instrument serial number located? The serial number is on. Devices SpectraMax® M4). Published Dec 16, 2020 | Updated Dec 16, 2020. Transfer plate to SpectraMax M5 with excitation monochromator set to 455 nm with 10 nm bandwidth, emission monochromator set to 525 nm with 10 nm bandwidth, and 515 nm cutoff filter. The conjugate was also characterized using FTIR (FT/IR-4200, Jasco Inc. Table 2. 2 only). However, traditional polyacrylamide gel electrophoresis (PAGE) and high. 3, La Jolla, CA, USA). org) with optimized instrument settings (Table 1) were used to generate and analyze data. The test conditions are as follows: mobile phase: acetonitrile/H2O (0. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Detection cartridge modularity allows for easy addition of new read modes within minutes without. AM (~0. After preparation, wrap in foil (see Note 1). The M3/M4/M5/M5e/Flex3 and the L/LMax II/LMax II 384 groups of readers each require different Dead Time Correction values, and therefore each has their own Certification. New PBS 1X was added into each well and oxidized DCFH excitation and emission were measured at 485 and 525 nm respectively in a spectrophotometer (SpectraMax M4, Molecular Devices, Sunnyvale, USA). 23, SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 23 PathCheck Pathlength Measurement Technology and Interfering Substances Any material that absorbs in the 900 nm to 1000 nm spectral region could interfere with PathCheck Pathlength Measurement Technology measurements. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, AquaMax washer, SpectraMax Plus 384 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax L reader, SpectraMax M5/M5e. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Titration of human serum and plasma (heparin) within the working range of the assay. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. High tolerance for contaminants. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM SpectraMax M4 Multi-Mode Microplate Reader. 1. The guide also covers the software features, the specifications, and the accessories of the systems. Cell viability was calculated using the formula as follows: (OD450 KO HepG2 -OD450 blank /OD450 WT HepG2 -OD450 blank ) × 100%. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Choose from a three- (M3), four- (M4), or Qualify the luminescence performance of the SpectraMax . VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Absorbance was read at 570 nm with SpectraMax M4 microplate spectrophotometer (Molecular Devices, CA). Eos - M4/3 lens adapter - Lenses - Victoria, British Columbia | Facebook. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. 75 × 10 5) suspended in PBS in 96-well dark plates incubated with NP or SiO 2 for 1 h at 37 °C. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. FlexStation ®, SpectraMax. 1. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate Reader The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 303 unable to cal dark current. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. , USA). 2. Standard curves for quantifying samples were generated by using serial dilutions of BSA from 0 to 0. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. NCI-60 Human Tumor Cell Line Screen. Polybead® nanoparticles were analyzed. Then cells were collected, and the caspase-3 expression level was detected with the caspase-3 assay kit. Examples: “Press carefully to engage,” “ Do not. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader 5 B > 4 4 5 B 5 : F 8 8 > 3 ; > I 5 = 8 5. Ninety microliters of 2-chloro-4-nitrophenyl-α- d -maltotrioside substrate dilutions from 5000 to 1. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. 1 Measurement of Intestinal Permeability (In Vivo). 2. Microplate Reader Shelf. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. Results. D 1 1. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. The drawing shows a standard microplate of 15 mm height covered with a lid. . 7. 22 μM was mixed with 10-μl enzyme giving a final enzyme concentration in the. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening.